Coding

Part:BBa_K4488005:Design

Designed by: Sylvie Nolf   Group: iGEM22_Sydney_Australia   (2022-09-30)


Anti-GFP Nanobody/sdAb


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Optimised codon usage for Escherichia coli. Removed commonly used and iGEM restriction sites. Added associated Esp3I site for usage


Source

Adapted from: http://doi.org/10.2210/pdb3OGO/pdb

References